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| Questions regarding antibodies | |
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| 1. | In which applications can I employ tag-tools antibodies? |
 Our antibodies have proven their reliability in a range of different applications.You will find more information in the product sheets, where validated applications and the used range of antibody concentrations is detailed. In all cases, our antibodies have been employed in Western Blotting, in immunoprecipitation and in immunofluorescence staining . If the product sheets lack detailed information about specific methods, such as Dot Blotting or immunohistochemistry, it does not imply that the antibodies can not be used for these kinds of analyses.  |
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| 2. | Do the antibodies recognize amino-terminal as well as carboxy-terminal epitope-tags? |
| Our antibodies detect their epitope in different configurations, including amino-terminal and carboxy-terminal extensions of proteins. Even embedded in a protein sequence, epitope-tags can be recognized successfully. It is important to consider, that to allow the detection of the native, non-denatured protein (e.g. during immunoprecipitation ) the epitope-tag has to be accessible for the antibody. Once the protein is denatured (e.g. during Western Blotting ), the position of the epitope-tag does not seem to be critical for antibody binding. |
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| 3. | Will epitope tags embedded within the protein sequence be detected by your antibodies? |
| Even embedded in a protein sequence, epitope-tags can be recognized successfully. It is important to consider, that to allow the detection of the native, non-denatured protein (e.g. during immunoprecipitation ) the epitope-tag has to be accessible for the antibody. Once the protein is denatured (e.g. during Western Blotting ), the position of the epitope-tag does not seem to be critical for antibody binding. |
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| 4. | Can your antibodies be used in flow cytometry experiments? |
| In principle, tag-tools antibodies can be employed for staining cells, which are to be analysed by flow cytometry. However, if the epitope-tag is not located outside of the cell, cellular membranes have to be permeabilized prior to staining. |
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| 5. | Do you provide epitope-tag antibodies with covalently attached fluorescent dyes or enzymes? |
| No. At the moment we offer our antibodies in a non-conjugated format only. In combination with a range of covalently coupled secondary reagents, this allows for maximum flexibility in the use of tag-tools antibodies in your experimental setups. |
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| 6. | Which secondary reagents do you recommend to be used in combination with tag-tools antibodies? |
| Secondary antibodies with different species specificity and a range of covalently attached detection modules (e.g. coupled to biotin, fluorochromes, or enzymes) are offered by a range of established suppliers. If you intend to combine several distinct antibodies (e.g. in double- or triple-immunofluorescence stainings), then it is instrumental that your secondary reagents have the lowest possible cross-reactivity with antibodies of other species. Often, this requires pre-adsorption with sera or antibodies from other species. |
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| 7. | Will you provide antibodies in bulk amounts? |
| Our packaging sizes are optimized for the use of the antibodies in standard research labs. Of course, we can also satisfy larger demands. Please get in touch with us to customize your XXL order. |
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