navigation: home > Protokolle
 Method |
download | |
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| 01 | Immunofluorescence staining | |
| Staining of fixed cells with primary monoclonal antibodies directed against a protein tag of interest and fluorescence labeled secondary reagents. |
IF Staining.pdf  |
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| 02 | Western Blot | |
| Detection of tagged proteins after electrophoretic separation and blotting to a membrane by primary monoclonal antibodies and horseradish-peroxidase-coupled secondary reagents. |
Western Blot.pdf |
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| 03 | Staining of cells for flow cytometry | |
| Staining of surface proteins with primary monoclonal antibodies directed against a protein tag of interest and fluorescence labeled secondary reagents. |
Flow Cytometry.pdf |
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| 04 | Immunoprecipitation | |
| Isolation of tagged proteins from complex protein mixtures, such as a whole cell lysate, by a tag-directed antibody and ProteinA- or ProteinG-coupled beads. |
Immunoprecipitation.pdf |
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